Domain 7.6Phlebotomy

Lab Operations And Reporting

Last updated June 16, 2026

Overview

This section merges Quality Control (QC), Departmental Organization, and Results Distribution. It covers how the lab is structured, how to ensure your equipment is accurate before testing, and how to handle the results after testing.


1. Laboratory Departments

Knowing which department processes a specific test is a common exam topic. The tube color often hints at the department.

Department Focus Common Tests Key Tubes
Hematology Study of blood and clotting. CBC, Coagulation Studies (PT, PTT), ESR. Lavender, Light Blue
Chemistry Chemical analysis of serum/plasma. Glucose, Cholesterol, BMP, CMP. Red, Gold, Green, Gray
Microbiology Study of microorganisms. Blood Cultures, Urine Cultures. Yellow, Culture Bottles
Serology Study of antibodies/immunity. Mononucleosis, HIV, Pregnancy. Red, SST

Exam Tip: Even though coagulation involves clotting factors, Coagulation studies are typically performed in the Hematology section (because "Hema" means blood).


2. Selected Manual Test Procedures

While most testing is automated, MAs must understand the setup for certain manual tests.

Erythrocyte Sedimentation Rate (ESR)

  • Purpose: Measures inflammation in the body.
  • Tubes Used: Westergren or Wintrobe tubes.
  • Procedure: The blood is placed in a tall, thin tube. The distance the red blood cells fall (sediment) in one hour is measured.
  • Critical Setup: The initial fill line for the blood in the tube is the 0mm mark at the very top. You measure from 0 downward.

3. Quality Control (QC)

QC ensures that test results are accurate and reliable. You must perform QC on CLIA-waived tests (like glucometers) and lab instruments.

A. Calibration vs. Controls

  • Calibration: Configuring an instrument to a known standard. It corrects the machine.
  • Controls: Testing samples with known values (High/Low) to verify the system is working.

B. Expected vs. Unexpected Results

  • Expected: Control value is within range. Action: Proceed with patient testing.
  • Unexpected (Out of Range): Control value is failed.
    • Action: STOP. Do not test patients. Troubleshoot (check expiration dates, storage, re-run). Document corrective actions.

C. The QC Log

Documentation is mandatory. You must log control results, expiration dates, lot numbers, and any corrective actions taken.


4. Results Distribution

Once the lab generates a result, you must triage it based on clinical significance.

A. Categories of Results

  1. Normal: Attach to chart for provider review.
  2. Abnormal: Flag for provider attention.
  3. Critical / "Panic" Value: Indicates a potentially life-threatening condition.

B. The Critical Value Protocol

Exam Scenario: You receive a Critical Value (e.g., extremely low hemoglobin).

  1. Action: Notify the Provider IMMEDIATELY. This is your #1 priority.
  2. Document: Record who you spoke to, the time, the value, and the instructions given.

C. Reference Laboratories

When sending tests to an outside (reference) lab:

  • Source of Truth: Use the Laboratory's Online Test Directory. It provides the most current instructions for tube type, volume, and transport.
  • Requisition: Must accompany the specimen.

5. Final Key Takeaways

  • Departments: Coagulation (PT/PTT) is part of Hematology.
  • ESR: Fill Westergren/Wintrobe tubes to the 0mm mark.
  • QC Failure: If controls fail, STOP. Do not report patient results.
  • Calibration: Sets the machine; Controls verify the process.
  • Critical Values: Require Immediate Provider Notification.
  • Reference Labs: Check the online directory for instructions.